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nuclear-localized yellow fluorescent protein (yfp  (Becton Dickinson)
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Becton Dickinson nuclear-localized yellow fluorescent protein (yfp
Overexpression of parkin <t>reduces</t> <t>A30P</t> α-synuclein-induced dopamine nigral neuron loss. (A) TH expression at 6 weeks in the substantia nigra of rats injected with different mixes of lentiviral vectors encoding for <t>YFP</t> (YFP2×), rat parkin (Parkin), and A30P human α-synuclein (A30P). (B) Histograms represent the loss of TH-IR nigral neurons at 6 weeks relative to the contralateral side in rats unilaterally injected with the different lentiviral constructs. Values refer to means ± SEM; n = 5 animals for YFP2× or parkin; n = 10 for A30P/YFP; n = 18 for A30P/parkin; *, P < 0.05, compared with lenti-YFP-injected animals; §, P < 0.005, compared with A30P/YFP-expressing animals. (Scale bar: 350 μm.)
Nuclear Localized Yellow Fluorescent Protein (Yfp, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Overexpression of parkin reduces A30P α-synuclein-induced dopamine nigral neuron loss. (A) TH expression at 6 weeks in the substantia nigra of rats injected with different mixes of lentiviral vectors encoding for YFP (YFP2×), rat parkin (Parkin), and A30P human α-synuclein (A30P). (B) Histograms represent the loss of TH-IR nigral neurons at 6 weeks relative to the contralateral side in rats unilaterally injected with the different lentiviral constructs. Values refer to means ± SEM; n = 5 animals for YFP2× or parkin; n = 10 for A30P/YFP; n = 18 for A30P/parkin; *, P < 0.05, compared with lenti-YFP-injected animals; §, P < 0.005, compared with A30P/YFP-expressing animals. (Scale bar: 350 μm.)

Journal:

Article Title: Lentiviral vector delivery of parkin prevents dopaminergic degeneration in an ?-synuclein rat model of Parkinson's disease

doi: 10.1073/pnas.0405313101

Figure Lengend Snippet: Overexpression of parkin reduces A30P α-synuclein-induced dopamine nigral neuron loss. (A) TH expression at 6 weeks in the substantia nigra of rats injected with different mixes of lentiviral vectors encoding for YFP (YFP2×), rat parkin (Parkin), and A30P human α-synuclein (A30P). (B) Histograms represent the loss of TH-IR nigral neurons at 6 weeks relative to the contralateral side in rats unilaterally injected with the different lentiviral constructs. Values refer to means ± SEM; n = 5 animals for YFP2× or parkin; n = 10 for A30P/YFP; n = 18 for A30P/parkin; *, P < 0.05, compared with lenti-YFP-injected animals; §, P < 0.005, compared with A30P/YFP-expressing animals. (Scale bar: 350 μm.)

Article Snippet: The cDNAs encoding nuclear-localized yellow fluorescent protein (YFP) (BD Biosciences Clontech), A30P human α-synuclein, and rat parkin were cloned into the SIN-W-PGK lentiviral transfer vector, and the viral particles (lenti-YFP, lenti-A30P, and lenti-parkin) were produced as described in refs. 24 and 30 .

Techniques: Over Expression, Expressing, Injection, Construct

Parkin prevents the α-synuclein-induced dopaminergic fiber loss in the striatum of rats. (A) Striatal sections stained for the TH marker from rats that received intranigral injection of rats injected with different solutions of lentiviral vectors encoding for YFP (YFP2×), rat parkin (Parkin), and A30P human α-synuclein (A30P). (B) Histograms represent the loss of TH-IR fibers neurons at 6 weeks relative to the contralateral side in rats unilaterally injected with the different lentiviral suspensions. Animals injected with lenti-A30P/lenti-YFP showed a considerable reduction of dopaminergic innervation on the ipsilateral side of the striatum. Values refer to means ± SEM; n = 5 animals for YFP2× or parkin; n = 12 for A30P/YFP; n = 13 for A30P/parkin; *, P < 0.05, compared with lenti-YFP2×-injected animals; §, P < 0.005, compared with A30P/YFP-expressing animals.

Journal:

Article Title: Lentiviral vector delivery of parkin prevents dopaminergic degeneration in an ?-synuclein rat model of Parkinson's disease

doi: 10.1073/pnas.0405313101

Figure Lengend Snippet: Parkin prevents the α-synuclein-induced dopaminergic fiber loss in the striatum of rats. (A) Striatal sections stained for the TH marker from rats that received intranigral injection of rats injected with different solutions of lentiviral vectors encoding for YFP (YFP2×), rat parkin (Parkin), and A30P human α-synuclein (A30P). (B) Histograms represent the loss of TH-IR fibers neurons at 6 weeks relative to the contralateral side in rats unilaterally injected with the different lentiviral suspensions. Animals injected with lenti-A30P/lenti-YFP showed a considerable reduction of dopaminergic innervation on the ipsilateral side of the striatum. Values refer to means ± SEM; n = 5 animals for YFP2× or parkin; n = 12 for A30P/YFP; n = 13 for A30P/parkin; *, P < 0.05, compared with lenti-YFP2×-injected animals; §, P < 0.005, compared with A30P/YFP-expressing animals.

Article Snippet: The cDNAs encoding nuclear-localized yellow fluorescent protein (YFP) (BD Biosciences Clontech), A30P human α-synuclein, and rat parkin were cloned into the SIN-W-PGK lentiviral transfer vector, and the viral particles (lenti-YFP, lenti-A30P, and lenti-parkin) were produced as described in refs. 24 and 30 .

Techniques: Staining, Marker, Injection, Expressing

Parkin prevents A30P α-synuclein-induced neurodegeneration. Brain sections from animals expressing A30P/YFP (A–C) or A30P/parkin (D–F) were stained for α-synuclein. A similar expression of A30P human α-synuclein was observed in both groups. No α-synuclein staining was observed for the noninjected side (NI), lenti-YFP-injected, or lenti-parkin-injected (data not shown) animals. Silver staining (B, C, and E–I) was performed on adjacent nigral sections to detect degenerating neurons. Higher magnification shows the presence of silver-positive dark structures in both cell body and axon of nigral neurons from animals expressing A30P/YFP (C). Coexpression of parkin with A30P α-synuclein prevents the appearance of silver-positive degenerating neurons (F). Noninjected side (NI) (G) and animals expressing YFP (H) or parkin (I) did not show any specific silver staining. (Scale bars: A, B, D, E, and G–I, 140 μm; C and F, 40 μm.)

Journal:

Article Title: Lentiviral vector delivery of parkin prevents dopaminergic degeneration in an ?-synuclein rat model of Parkinson's disease

doi: 10.1073/pnas.0405313101

Figure Lengend Snippet: Parkin prevents A30P α-synuclein-induced neurodegeneration. Brain sections from animals expressing A30P/YFP (A–C) or A30P/parkin (D–F) were stained for α-synuclein. A similar expression of A30P human α-synuclein was observed in both groups. No α-synuclein staining was observed for the noninjected side (NI), lenti-YFP-injected, or lenti-parkin-injected (data not shown) animals. Silver staining (B, C, and E–I) was performed on adjacent nigral sections to detect degenerating neurons. Higher magnification shows the presence of silver-positive dark structures in both cell body and axon of nigral neurons from animals expressing A30P/YFP (C). Coexpression of parkin with A30P α-synuclein prevents the appearance of silver-positive degenerating neurons (F). Noninjected side (NI) (G) and animals expressing YFP (H) or parkin (I) did not show any specific silver staining. (Scale bars: A, B, D, E, and G–I, 140 μm; C and F, 40 μm.)

Article Snippet: The cDNAs encoding nuclear-localized yellow fluorescent protein (YFP) (BD Biosciences Clontech), A30P human α-synuclein, and rat parkin were cloned into the SIN-W-PGK lentiviral transfer vector, and the viral particles (lenti-YFP, lenti-A30P, and lenti-parkin) were produced as described in refs. 24 and 30 .

Techniques: Expressing, Staining, Injection, Silver Staining

Parkin increases the number of phosphorylated α-synuclein inclusions. Sections from the substantia nigra of rats expressing A30P α-synuclein and parkin were immunostained with the Pser129 Ab specific for phospho-Ser-129 of α-synuclein that selectively and extensively accumulates in human Lewy bodies. (A) A very weak Pser129 staining was observed in the noninjected side corresponding to the physiological level of phosphorylated rat α-synuclein. (B) The substantia nigra of animals overexpressing either A30P/YFP or A30P/parkin revealed the presence of numerous hyperphosphorylated inclusions reminiscent of Lewy bodies. (C) Pser129-positive neurites were occasionally observed in animals overexpressing A30P/parkin. (D) Triple staining with Pser129 Ab (green), LB509 human α-synuclein specific Ab (red), and TOPRO-3 nuclear marker (blue) shows that phosphorylated inclusions abundantly contain human α-synuclein. (E) The number of neurons containing Pser129-positive inclusions were quantified in the substantia nigra of rats expressing A30P/YFP or A30P/parkin. Values refer to means ± SEM; n = 6 animals per group; *, P < 0.05; **, P < 0.005. (Scale bars: A and B, 150 μm; C, 40 μm; D, 10 μm.)

Journal:

Article Title: Lentiviral vector delivery of parkin prevents dopaminergic degeneration in an ?-synuclein rat model of Parkinson's disease

doi: 10.1073/pnas.0405313101

Figure Lengend Snippet: Parkin increases the number of phosphorylated α-synuclein inclusions. Sections from the substantia nigra of rats expressing A30P α-synuclein and parkin were immunostained with the Pser129 Ab specific for phospho-Ser-129 of α-synuclein that selectively and extensively accumulates in human Lewy bodies. (A) A very weak Pser129 staining was observed in the noninjected side corresponding to the physiological level of phosphorylated rat α-synuclein. (B) The substantia nigra of animals overexpressing either A30P/YFP or A30P/parkin revealed the presence of numerous hyperphosphorylated inclusions reminiscent of Lewy bodies. (C) Pser129-positive neurites were occasionally observed in animals overexpressing A30P/parkin. (D) Triple staining with Pser129 Ab (green), LB509 human α-synuclein specific Ab (red), and TOPRO-3 nuclear marker (blue) shows that phosphorylated inclusions abundantly contain human α-synuclein. (E) The number of neurons containing Pser129-positive inclusions were quantified in the substantia nigra of rats expressing A30P/YFP or A30P/parkin. Values refer to means ± SEM; n = 6 animals per group; *, P < 0.05; **, P < 0.005. (Scale bars: A and B, 150 μm; C, 40 μm; D, 10 μm.)

Article Snippet: The cDNAs encoding nuclear-localized yellow fluorescent protein (YFP) (BD Biosciences Clontech), A30P human α-synuclein, and rat parkin were cloned into the SIN-W-PGK lentiviral transfer vector, and the viral particles (lenti-YFP, lenti-A30P, and lenti-parkin) were produced as described in refs. 24 and 30 .

Techniques: Expressing, Staining, Marker